|
KMID : 0377619880530070425
|
|
Korean Jungang Medical Journal
1988 Volume.53 No. 7 p.425 ~ p.432
|
|
|
Biochemical Studies on the Defective Step of Cholesterol Biosynthetic Pathway of Mouse Myeloma Cells
|
|
金秀福/Kim, S.B.
李仁洙/崔喜雄/曺永俊/李秀寬/崔東洙/Lee, I.S./Choi, H.W./Cho, Y.J./Lee, S.K./Choi, D.S.
|
|
|
Abstract
|
|
|
The biochemical basis of defective step of cholesterol biosynthetic pathway for the cholesterol-dependent growth phenotype of the mouse myeloma, cell line has been studied.
In this studies, a cholesterol-dependent growth phenotype and a cholesterol independent growth variant of. mouse myeloma cells were labelled with [2-^14C] acetate and the distributions of radioactivity between cholesterol and its precursors were determined by thin layer chromatography.
The major labelled sterol product in the mouse myeloma cells after a 24 hr exposure to[2-14C] acetate was lanosterol and the major labelled sterol product in the variant cells after a 24 hr exposure to [2-14 C] acetate was cholesterol.
These results indicate that mouse myeloma cells are defective in cholesterol biosynthetic pathway and the site of defective is the step of.demethylation of lanosterol to C-29 sterol internediates.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|